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Bioanalysis of complex biotherapeutics, such as antibody-drug conjugates ADCsis challenging and requires multiple assays to describe their pharmacokinetic PK profiles. MEDI is an ADC comprised of biparatopic humanized antibody attached via a protease-cleavable peptide-based maleimidocaproyl linker to a tubulysin toxin AZ with an approximate average drug-antibody ratio of 4. In this report, we describe the development, validation and application of three novel multiplex bioanalytical methods.

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